Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An rRNA variable region has an evolutionarily conserved essential role despite sequence divergence

R Sweeney1, L Chen, M C Yao

  • 1Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

Molecular and Cellular Biology
|June 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Covalent linkage between proteins of the inter-alpha-inhibitor family and hyaluronic acid is mediated by a factor produced by granulosa cells.

The Journal of biological chemistry·1996
Same author

Two-tone rate suppression boundaries of cochlear ganglion neurons in normal chickens.

The Journal of the Acoustical Society of America·1996
Same author

Greater ozone-induced inflammatory responses in subjects with asthma.

American journal of respiratory and critical care medicine·1996
Same author

Use of combinatorial peptide libraries to construct functional mimics of tumor epitopes recognized by MHC class I-restricted cytolytic T lymphocytes.

The Journal of experimental medicine·1996
Same author

Recurrent transition at a CG dinucleotide in exon 12 of COL2A1 produces kniest dysplasia with abnormal RNA splicing by chondrocytes and lymphoblasts and interruption of the triple helix of type II collagen.

Annals of the New York Academy of Sciences·1996
Same author

Inogatran, a novel direct low molecular weight thrombin inhibitor, given with, but not after, tissue-plasminogen activator, improves thrombolysis.

The Journal of pharmacology and experimental therapeutics·1996

Deleting the D8 region of large-subunit ribosomal RNA (rRNA) in Tetrahymena thermophila inactivates rRNA genes, preventing growth. However, replacing it with D8 regions from other organisms restores function, suggesting structural, not sequence, similarity is key.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Eukaryotic ribosomal RNAs (rRNAs) contain highly variable regions at conserved locations.
  • The functional significance of these variable regions remains largely unexplored.

Purpose of the Study:

  • To investigate the functional importance of the D8 region in Tetrahymena thermophila large-subunit rRNA.
  • To determine if sequence variability or structural constraints dictate the function of the D8 region.

Main Methods:

  • Gene reconstruction and replacement experiments were performed in Tetrahymena thermophila.
  • Deletion and substitution of the D8 rRNA region were analyzed.
  • Functional assessment of modified rRNA genes was conducted through growth assays and analysis of rRNA processing.

Related Experiment Videos

Main Results:

  • Deletion of the D8 region completely inactivated Tetrahymena thermophila rRNA genes, leading to growth arrest.
  • Replacement with unrelated sequences or variable regions from other rRNA positions also resulted in inactivation.
  • Functional restoration was achieved by replacing the D8 region with homologous regions from three different organisms, despite significant sequence divergence.
  • Mutant rRNAs accumulated as precursor forms, indicating defects in processing or stabilization.

Conclusions:

  • The D8 region is essential for the function of Tetrahymena thermophila large-subunit rRNA.
  • Functional interchangeability of D8 regions from different organisms suggests a conserved tertiary structure, rather than primary sequence, is critical for its role.
  • This highlights the importance of higher-order RNA structure in essential cellular processes.