Related Experiment Video
Updated: Jul 13, 2026
10:15
Capturing Chromosome Conformation Across Length Scales
Published on: January 20, 2023
Complete DNA sequence of yeast chromosome XI
B Dujon1, D Alexandraki, B André
1Unité de Génétique Moléculaire des Levures (URA 1149 du CNRS and UFR927 University P.M. Curie), Départment de Biologie Moléculaire, Insitut Pasteur, Paris, France.
Nature
|June 2, 1994
Summary
The complete DNA sequence of yeast chromosome XI was determined, revealing patterns in base composition and gene density. This finding highlights the need for accurate physical mapping of chromosomes.
Area of Science:
- Genomics
- Molecular Biology
- Yeast Genetics
Background:
- The Saccharomyces cerevisiae genome is a model for eukaryotic chromosome structure.
- Understanding chromosome organization is crucial for deciphering gene regulation and function.
Purpose of the Study:
- To determine the complete DNA sequence of Saccharomyces cerevisiae chromosome XI.
- To identify general chromosome patterns and their relationship with gene density.
- To assess the accuracy of existing genetic maps against physical mapping data.
Main Methods:
- High-throughput DNA sequencing to determine the 666,448-base-pair sequence of chromosome XI.
- Bioinformatic analysis to identify protein coding sequences and analyze base composition.
- Comparison of the determined physical map with the previously published genetic map.
Main Results:
- The complete DNA sequence of chromosome XI was successfully determined.
- Alternating regional variations in average base composition were observed, correlating with local gene density.
- Significant discrepancies were found between the determined physical map and the existing genetic map.
Conclusions:
- The DNA sequence of Saccharomyces cerevisiae chromosome XI provides a comprehensive physical map.
- Chromosome structure exhibits regional variations in base composition linked to gene distribution.
- Physical mapping is essential for accurate chromosome representation, necessitating a re-evaluation of genetic mapping techniques.
Related Concept Videos
01:17Karyotyping
Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...