[Analysis by flow cytometry and cell sorting. Report of current status and perspectives for pathology]

Although flow cytometry was initially used just to count cells, it has developed into a method of measuring the physical and chemical properties of individual cells when they are detected in a fluid stream by electronic sensors. With the application of fluorescence molecules, especially fluorescence-conjugated antibodies that are excited by focused laser light, the spectrum of application has expanded and has for years exceeded the traditional field of hematology. The article presents applications for pathology with the main focus on oncological problems such as DNA multiparameter analysis and proliferation assessment. In addition, the basic aspects of the different types of user-friendly flow cytometers are explained here.