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Updated: Aug 11, 2026

HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
Published on: July 9, 2016
The heterologous expression and characterization of human prostaglandin G/H synthase-2 (COX-2)
D B Miller1, D Munster, J S Wasvary
1Department of Biological Technologies, Ciba Pharmaceuticals Division, CIBA-GEIGY Corporation, Summit, NJ 07901.
Abstract:
The open reading frame of human cyclooxygenase-2 was cloned by pcr amplification of IL-1 beta stimulated human dermal fibroblast cDNA. The coding region was used to construct a recombinant baculovirus which when used to infect Sf9 cells directed the expression of recombinant human cyclooxygenase-2. The heterologously expressed enzyme was characterized and found to display all salient features of cyclooxygenase. Large-scale microsomal preparations of infected cells yielded more than 20 units of enzyme with a specific activity of 240 nmoles prostaglandin product/mg protein.

