Influence of perioperative storage solutions on long-term vein graft function and morphology

It has been shown that suboptimal preparation of a vein graft prior to its insertion results in immediate morphologic and functional damage to the endothelial cells but not to the underlying smooth muscle cells. However, little is known about whether such perioperative injury to the vein grafts influences the subsequent development of intimal hyperplasia and smooth muscle cell contractility. This study examines the influence of storage in saline solution or Ringer's lactate on the development of intimal hyperplasia and vasomotor function in experimental vein grafts. Twenty-six New Zealand white rabbits had a carotid vein bypass graft performed after the veins had been immersed (15 minutes) in either heparinized saline solution (Sal; n = 13) or Ringer's lactate (RL; n = 13), and each group was harvested after 28 days for either histologic (n = 8) or functional studies (n = 5; four 5 mm rings/graft). Saline storage of the vein graft resulted in a 38% increase in the thickness of the intimal hyperplasia (113 +/- 2 vs. 83 +/- 2 microns, Sal vs. RL; mean +/- SEM; p < 0.05) without a change in medial thickness (87 +/- 5 vs. 86 +/- 8 microns, Sal vs. RL; p > 0.05). The two sets of vein grafts showed no difference in sensitivity to norepinephrine, serotonin, and bradykinin.(ABSTRACT TRUNCATED AT 250 WORDS)