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Related Experiment Videos

p53 abnormalities in human parathyroid carcinoma

V L Cryns1, M P Rubio, A D Thor

  • 1Endocrine Oncology Laboratory, Massachusetts General Hospital, Boston 02114.

The Journal of Clinical Endocrinology and Metabolism
|June 1, 1994
PubMed
Summary

The p53 tumor suppressor gene, a key cell cycle regulator, shows abnormalities in parathyroid carcinomas, including allelic loss and abnormal protein expression. These findings suggest p53

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Area of Science:

  • Oncology
  • Molecular Genetics
  • Cell Biology

Background:

  • Parathyroid neoplasms involve cell cycle regulators like PRAD1/cyclin D1 and retinoblastoma gene.
  • The role of the p53 tumor suppressor gene in parathyroid tumor pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate molecular genetic abnormalities of the p53 tumor suppressor gene in parathyroid adenomas and carcinomas.
  • To determine the potential involvement of p53 in the development of parathyroid neoplasms.

Main Methods:

  • Analysis of p53 gene allelic loss in parathyroid tumors.
  • Immunohistochemical detection of nuclear p53 protein.
  • Single strand conformation polymorphism (SSCP) analysis of p53 exons 5-9.

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Main Results:

  • p53 allelic loss was detected in 2 of 6 informative parathyroid carcinomas.
  • Abnormal nuclear p53 protein was found in 2 of 9 parathyroid carcinomas.
  • No p53 allelic loss was observed in 20 informative parathyroid adenomas; one adenoma showed focal p53 protein staining.
  • No mutations in p53 exons 5-9 were identified in any parathyroid neoplasm.

Conclusions:

  • p53 allelic loss and abnormal p53 protein expression implicate the p53 gene in the pathogenesis of a subset of parathyroid carcinomas.
  • The study suggests that p53 mutations in parathyroid tumors, if present, may occur outside of the analyzed exons.