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Related Experiment Videos

Complement activation in platelet concentrates is surface-dependent and modulated by the platelets

M I Gyongyossy-Issa1, E McLeod, D V Devine

  • 1Canadian Red Cross Society Blood Services, Vancouver Centre, Canada.

The Journal of Laboratory and Clinical Medicine
|June 1, 1994
PubMed
Summary

Complement activation contributes to platelet storage lesion. Complement activation via the classical pathway occurred during storage, with higher levels in platelet-poor plasma. Platelets showed complement deposition and increased decay accelerating factor expression.

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Area of Science:

  • Immunology
  • Transfusion Medicine
  • Biochemistry

Background:

  • Platelet storage lesion (PSL) impacts platelet function and viability.
  • Complement activation is a potential mediator of PSL.
  • Understanding PSL mechanisms is crucial for improving blood product storage.

Purpose of the Study:

  • Investigate the role of complement activation in PSL.
  • Monitor complement activation fragments during platelet storage.
  • Assess the influence of platelets and storage surfaces on complement pathways.

Main Methods:

  • Studied platelet bags stored up to 5 days.
  • Monitored complement activation fragments (classical and alternative pathways).
  • Used flow cytometry to detect complement components on platelet surfaces.

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Main Results:

  • C4d, C3a, and SC5b-9 levels increased during storage.
  • Complement generation was higher in platelet-poor plasma.
  • Platelet-associated C3 and decay accelerating factor expression increased during storage.

Conclusions:

  • Complement activation, primarily via the classical pathway, occurs during platelet storage.
  • Platelets and storage surfaces influence complement activation patterns.
  • Complement activation may contribute to the platelet storage lesion.