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Related Experiment Videos

Development of a mouse model for nonbacterial prostatitis

D W Keetch1, P Humphrey, T L Ratliff

  • 1Division of Urologic Surgery, Washington University School of Medicine, St. Louis, Missouri 63141.

The Journal of Urology
|July 1, 1994
PubMed
Summary

Researchers developed an animal model for nonbacterial prostatitis by injecting prostate tissue into mice. This study suggests nonbacterial prostatitis may be an immune-mediated autoimmune condition.

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Area of Science:

  • Immunology
  • Urology
  • Pathology

Background:

  • Nonbacterial prostatitis is a prevalent clinical condition with poorly understood etiology and pathogenesis.
  • Diagnosis and treatment of nonbacterial prostatitis remain challenging.
  • Developing an animal model is crucial for studying the immune parameters of this disease.

Purpose of the Study:

  • To establish an animal model for nonbacterial prostatitis.
  • To characterize the immune parameters involved in nonbacterial prostatitis.
  • To investigate the potential autoimmune nature of nonbacterial prostatitis.

Main Methods:

  • Prostates were harvested from various mouse strains (SJL, AJ, Balb/c, C57bl/6, C57bl/6 lpr).
  • Homogenized prostate tissue was injected into syngeneic mice; controls received Freund's complete adjuvant.

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  • Prostatic inflammation was assessed histologically 30 days post-injection; adoptive transfer studies were performed.

    • Main Results:

    • Balb/c mice showed no inflammation; SJL and AJ mice exhibited varying degrees of inflammation.
    • C57bl/6 mice displayed lymphocytic infiltration in prostatic stroma and periglandular regions.
    • Prostatic inflammation was demonstrated to be at least partially immune-mediated.

    Conclusions:

    • Injection of syngeneic prostate antigen successfully induces prostatic inflammation resembling nonbacterial prostatitis.
    • Nonbacterial prostatitis may represent an autoimmune process.
    • This animal model facilitates further research into the pathogenesis and treatment of nonbacterial prostatitis.