Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

4-Dimethylaminophenethylamine, a sensitive, specific, electrochemically detectable monoamine oxidase-B substrate

M Reyes-Parada1, M C Scorza, R Silveira

  • 1Cell Biology Division, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.

Life Sciences
|January 1, 1994
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multitarget neuroprotection by quercetin: Changes in gene expression in two perinatal asphyxia models.

Neurochemistry international·2021
Same author

Acutely applied MDMA enhances long-term potentiation in rat hippocampus involving D1/D5 and 5-HT2 receptors through a polysynaptic mechanism.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology·2012
Same author

Preclinical and clinical characterization of the selective 5-HT(1A) receptor antagonist DU-125530 for antidepressant treatment.

British journal of pharmacology·2011
Same author

Fasciculin, a powerful anticholinesterase polypeptide from Dendroaspis angusticeps venom.

Neurochemistry international·2010
Same author

In vivo effects of 3-iodocytisine: pharmacological and genetic analysis of hypothermia and evaluation of chronic treatment on nicotinic binding sites.

Neuropharmacology·2009
Same author

Differentiation induced by Achyrocline satureioides (Lam) infusion in PC12 cells.

Phytotherapy research : PTR·2009

4-Dimethylaminophenethylamine (DMAPEA) is a selective MAO-B substrate, ideal for measuring enzyme activity. Its unique properties allow for sensitive detection in various biological samples using HPLC-ED.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Enzymology

Background:

  • Monoamine oxidase (MAO) enzymes are crucial in neurotransmitter metabolism.
  • MAO-A and MAO-B exhibit distinct substrate specificities and inhibitor sensitivities.
  • Developing selective substrates is vital for studying MAO activity in different tissues.

Purpose of the Study:

  • To characterize 4-Dimethylaminophenethylamine (DMAPEA) as a substrate for monoamine oxidase (MAO).
  • To evaluate the selectivity and kinetic properties of DMAPEA with MAO-A and MAO-B.
  • To establish DMAPEA as a tool for quantifying MAO-B activity.

Main Methods:

  • Enzyme kinetics assays using crude rat brain mitochondrial suspensions.
  • Oxidation of DMAPEA by MAO enzymes.

Related Experiment Videos

  • Electrochemical detection (ED) of DMAPEA and its oxidation product (DMAPAA) via High-Performance Liquid Chromatography (HPLC).

    • Main Results:

    • DMAPEA was found to be an MAO substrate, unaffected by MAO-A.
    • DMAPEA oxidation by MAO-B was linear with time and enzyme concentration.
    • Kinetic parameters for MAO-B: Km = 5.8 µM and Vmax = 21.2 pmol/min/mg protein.
    • Both DMAPEA and DMAPAA were electrochemically detectable at 0.85 V.

    Conclusions:

    • DMAPEA exhibits high affinity and selectivity for MAO-B.
    • Its low oxidation potential and detectability make it a unique tool for MAO-B activity determination.
    • DMAPEA facilitates sensitive MAO-B assays in diverse biological preparations using HPLC-ED.