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Microsatellite markers for genetic mapping in the chicken
1USDA, Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
Poultry Science
|April 1, 1994
Summary
Chicken microsatellite markers, specifically (TG)n repeats, were investigated for their polymorphism. These markers show utility for genotyping specific chicken populations, aiding genetic studies.
Area of Science:
- Animal Genetics
- Molecular Biology
- Genomics
Background:
- Microsatellite markers are abundant and polymorphic in eukaryotic genomes.
- The application of (TG)n microsatellites in chickens requires further investigation.
Purpose of the Study:
- To assess the utility of (TG)n microsatellites in the chicken genome.
- To identify polymorphic microsatellite markers for chicken genetic studies.
Main Methods:
- Generation of a chicken DNA library enriched for (TG)n repeats.
- Sequencing of 42 unique clones containing (TG)n microsatellites.
- Polymerase chain reaction (PCR) amplification and polymorphism screening using 33 primer sets across three chicken populations.
Main Results:
- Identified 42 unique (TG)n microsatellite clones with an average of 7.8 repeats.
- 19 out of 33 primer sets yielded polymorphisms in East Lansing (EL) and Compton (C) reference families and between Line 63 and Line 72.
- Polymorphic markers were genetically mapped in EL and C families, with 9 and 7 markers, respectively, showing polymorphism between Line 63 and Line 72.
Conclusions:
- Chicken (TG)n microsatellites are polymorphic and useful for genetic analysis.
- These microsatellite markers significantly enhance the ability to genotype specific loci in chicken populations.