Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specific T-cell tolerance may be preceded by a primary response

L Vidard1, L J Colarusso, B Benacerraf

  • 1Division of Lymphocyte Biology, Dana-Farber Cancer Institute, Boston, MA 02115.

Proceedings of the National Academy of Sciences of the United States of America
|June 7, 1994
PubMed
Summary

Ovalbumin induces T-cell tolerance in mice, marked by reduced interleukin-2 production. This tolerance is not solely due to anergy or suppression, suggesting T-cell refractoriness may be involved.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nobel laureates' letter to President Bush.

The Washington post·2002
Same author

Three-dimensional prenatal diagnosis of frontonasal malformation and unilateral cleft lip/palate.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2002
Same author

Isolated major congenital heart disease.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2001
Same author

Variation in fetal femur length with respect to maternal race.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine·2001
Same author

Isolated polydactyly: prenatal diagnosis and perinatal outcome.

Prenatal diagnosis·2000
Same author

The prognostic significance of hyperextension of the fetal head detected antenatally with ultrasound.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2000

Area of Science:

  • Immunology
  • T-cell tolerance induction
  • Allergen immunotherapy research

Background:

  • T-cell tolerance is crucial for preventing autoimmune diseases and managing allergies.
  • Understanding the mechanisms of T-cell tolerance is essential for developing effective immunotherapies.
  • Ovalbumin is a common model antigen used to study immune responses and tolerance.

Purpose of the Study:

  • To evaluate ovalbumin's efficacy in inducing T-cell-specific tolerance in SJL mice.
  • To investigate the underlying mechanisms of T-cell unresponsiveness in ovalbumin-tolerized mice.
  • To compare different tolerization routes (oral, intraperitoneal, subcutaneous) for ovalbumin.

Main Methods:

  • Induction of tolerance using ovalbumin via oral, intraperitoneal (i.p.), and subcutaneous (s.c.) routes in SJL mice.

Related Experiment Videos

  • Measurement of interleukin-2 (IL-2) production in lymph-node T-cell cultures upon antigen stimulation.
  • Adoptive transfer experiments using splenic or lymph-node T cells from tolerant to naive mice.

    • Main Results:

    • Ovalbumin successfully induced T-cell tolerance, evidenced by significantly decreased IL-2 production.
    • Oral tolerization was less effective than i.p. or s.c. routes.
    • T-cell transfer experiments ruled out suppression as the primary mechanism of tolerance.

    Conclusions:

    • Anergy alone cannot fully explain the observed T-cell unresponsiveness.
    • Programmed cell death is a possible, but not definitively proven, mechanism.
    • T-cell refractoriness is proposed as a potential explanation for reduced IL-2 production in tolerized mice.