Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitation of constant and variable region genes for mouse immunoglobulin lambda chains

T Honjo, S Packman

    Biochemistry
    |June 29, 1976
    PubMed
    Summary

    Researchers synthesized lambda light chain cDNA probes. These probes suggest a limited number of variable region genes, challenging the idea of unique genes for each lambda light chain variant.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Biochemical characteristics of newborns with carnitine transporter defect identified by newborn screening in California.

    Molecular genetics and metabolism·2017
    Same author

    Psychological aspects of patients with Fabry disease.

    Journal of inherited metabolic disease·2009
    Same author

    Phenotypic heterogeneity of N370S homozygotes with type I Gaucher disease: an analysis of 798 patients from the ICGG Gaucher Registry.

    Journal of inherited metabolic disease·2008
    Same author

    Improvement of bone disease by imiglucerase (Cerezyme) therapy in patients with skeletal manifestations of type 1 Gaucher disease: results of a 48-month longitudinal cohort study.

    Clinical genetics·2008
    Same author

    Executive functioning in children and adolescents with phenylketonuria.

    Clinical genetics·2007
    Same author

    Imiglucerase (Cerezyme) improves quality of life in patients with skeletal manifestations of Gaucher disease.

    Clinical genetics·2007

    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Cancer Research

    Background:

    • Myeloma tumors produce specific immunoglobulin light chains, such as lambda chains.
    • Understanding the genetic basis of immunoglobulin light chain diversity is crucial for immunology and cancer research.

    Purpose of the Study:

    • To synthesize and characterize cDNA complementary to lambda light chain mRNA.
    • To investigate the number of variable region genes encoding lambda light chains.
    • To assess the germ line hypothesis regarding immunoglobulin gene organization.

    Main Methods:

    • Synthesis of complementary DNA (cDNA) from myeloma tumor mRNA.
    • Characterization of cDNA length and sequence encoding lambda light chain regions.
    • Cross-hybridization studies using the lambda cDNA probe with various lambda subgroups.
    • Hybridization kinetic analysis to determine gene copy number in genomic DNA.

    Main Results:

    • Synthesized cDNA encodes significant portions of lambda light chain variable and constant regions.
    • The lambda cDNA probe cross-hybridizes with multiple lambda subgroups, including MOPC-315.
    • Analysis suggests a minimum of eight variable region genes for the lambda subgroup.
    • Genomic DNA analysis indicates approximately two copies of these sequences per haploid genome.

    Conclusions:

    • The findings challenge the notion of separate germ line genes for each individual lambda light chain variant.
    • A limited number of variable region genes likely account for lambda light chain diversity.
    • Further investigation is needed to address certain reservations regarding these conclusions.

    Related Experiment Videos