[Relation between nuclear DNA content and lymph node metastasis in submucosal early gastric cancer]

To investigate a possible relation between the nuclear DNA content and lymph node metastasis of submucosal early gastric cancer, DNA content was analyzed for 46 patients with lymph node metastasis and 67 patients without nodal metastasis. DNA aneuploidy was found in 20 (43.5%) of 46 patients with lymph node metastasis and in 31 (46.3%) of 67 patients without it. There was no statistical difference in the incidence of aneuploidy between the 2 groups. Among the cases with DNA diploidy, the mean value of S phase fraction was 6.82% in patients with lymph node metastasis and 5.65% in those without metastasis. The mean value of S phase fraction was significantly higher in patients with nodal involvement (p < 0.05). Furthermore, among the cases with DNA aneuploidy, the mean value of G2/M phase fraction was 11.03% in patients with lymph node metastasis and 7.54% in patients without metastasis. The mean value of G2/M phase fraction was significantly higher in patients with nodal involvement (p < 0.05). These findings suggest the significant value of the S and G2/M phase fraction for the prediction of lymph node metastasis in patients with submucosal early gastric cancer.