Circadian oscillation of 64-kDa polypeptide in the rat suprachiasmatic nucleus

In cell suspensions of suprachiasmatic nucleus harvested every 3 hr from rats kept under 12 hr: 12 hr light-dark cycle and constant darkness, we have detected a M(r) 64-kDa protein whose synthesis exhibits two distinct daily peaks in SDS-PAGE. Analysis of densitometer tracings revealed that the synthesis of other proteins was independent of the time of day or not reproducible. Maximum synthesis of the 64-kDa polypeptide occurred at around CT6 and CT21, which are almost coincident with the phase advance regions of circadian activity rhythm induced by anisomycin and light pulses [15], respectively. These results suggest that the 64-kDa protein in SDS-PAGE may be a part of the circadian clock mechanism.