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Related Experiment Videos

The never ripe mutation blocks ethylene perception in tomato

M B Lanahan1, H C Yen, J J Giovannoni

  • 1Agricultural Group, Monsanto Company, Chesterfield, Missouri 63198.

The Plant Cell
|April 1, 1994
PubMed
Summary

The tomato mutant Never ripe (Nr) shows ethylene insensitivity, blocking fruit ripening and flower senescence. This ethylene insensitivity is a single, codominant trait affecting multiple plant processes.

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Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Hormone Signaling

Background:

  • Ethylene is a key plant hormone regulating fruit ripening and senescence.
  • Tomato fruit ripening mutants are crucial for understanding ethylene's role.
  • The Never ripe (Nr) mutant exhibits a non-ripening phenotype.

Purpose of the Study:

  • To investigate the cause of the non-ripening phenotype in the Nr tomato mutant.
  • To determine the genetic basis and pleiotropic effects of ethylene insensitivity in tomato.
  • To compare the Nr mutant with plants engineered for altered ethylene biosynthesis.

Main Methods:

  • Screening of tomato fruit ripening mutants for ethylene response.
  • Segregation analysis to determine the inheritance pattern of ethylene insensitivity.

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  • Phenotypic characterization of Nr mutant and ACC synthase-overexpressing plants.

    • Main Results:

    • The Nr mutant displayed insensitivity to ethylene, unlike other mutants tested.
    • Ethylene insensitivity in Nr is a single, codominant, pleiotropic trait affecting flower senescence and abscission.
    • ACC synthase-overexpressing plants showed opposite phenotypes to Nr, with accelerated senescence and abscission.

    Conclusions:

    • The Nr mutant's non-ripening phenotype is due to insensitivity to ethylene.
    • Ethylene plays a critical role in regulating senescence and abscission in tomato flowers and petioles.
    • While ethylene is important for leaf senescence, other signals are also involved.