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Peripheral blood stem cell collection using the small volume collection chamber in the Fenwal CS-3000 Plus blood cell
C S Rosenfeld1, H Cullis, T Tarosky
1West Penn Hospital, Western Pennsylvania Cancer Institute, Pittsburgh 15224.
Abstract:
Optimal methods for peripheral blood stem cell (PBSC) collection should yield a small volume product containing minimal platelets and a large number of mononuclear cells (MNC). The Fenwal CS-3000 Plus blood cell separator was modified in an attempt to meet these objectives. Modifications of the CS-3000 Plus included use of the small volume collection chamber (SVCC), increasing the interface/offset detector setting to 150 and decreasing the centrifuge speed to 1400 rpm. Thirty-eight patients undergoing 224 PBSC collections were studied. Mobilization methods included 4 g/m2 cyclophosphamide (CY), CY + 250 micrograms/m2 subcutaneous granulocyte-macrophage colony-stimulating factor (GM-CSF) or GM-CSF alone. The median collection volume was 58 ml containing a median of 21 ml of red blood cells. Platelet collection efficiency was < 4.4% and the median number of extracted platelets was 0.6 x 10(11)/apheresis. Median reduction in the platelet count post-apheresis was 15%. MNC purity was 95.5% and MNC collection efficiency was 61%. Yield of MNC was 1 x 10(8)/kg/apheresis. Collected progenitor cells correlated with both the WBC and MNC content of the apheresis product. The modified CS-3000 Plus with the SVCC is effective for PBSC collection following three different mobilization regimens and is, therefore, recommended for routine collection of PBSC.
Insights
Modified Fenwal CS-3000 Plus effectively collects peripheral blood stem cells (PBSC), yielding a small volume with minimal platelets and high mononuclear cells (MNC). This optimized apheresis method is recommended for routine PBSC collection.
Area of Science:
- Hematology
- Transplant Medicine
- Biomedical Engineering
Background:
- Optimizing peripheral blood stem cell (PBSC) collection is crucial for transplant success.
- Current methods aim for small product volumes with high mononuclear cell (MNC) and low platelet yields.
- The Fenwal CS-3000 Plus is a commonly used blood cell separator.
Purpose of the Study:
- To modify the Fenwal CS-3000 Plus for improved PBSC collection efficiency.
- To evaluate the efficacy of a modified CS-3000 Plus system in collecting PBSC with specific product characteristics.
- To assess the impact of different mobilization regimens on PBSC collection outcomes.
Main Methods:
- Modification of the Fenwal CS-3000 Plus using a small volume collection chamber (SVCC), increased interface/offset detector setting (150), and decreased centrifuge speed (1400 rpm).
- Collection of PBSC from 38 patients undergoing 224 apheresis procedures.
- Evaluation of collection volume, red blood cell and platelet content, MNC purity, and MNC yield.
- Analysis of PBSC collection following mobilization with cyclophosphamide (CY), CY + granulocyte-macrophage colony-stimulating factor (GM-CSF), or GM-CSF alone.
Main Results:
- The modified system yielded a median collection volume of 58 ml with 21 ml of red blood cells.
- Platelet collection efficiency was < 4.4%, with a median of 0.6 x 10(11) platelets/apheresis, and a 15% median reduction in post-apheresis platelet count.
- Mononuclear cell (MNC) purity was 95.5%, MNC collection efficiency was 61%, and MNC yield was 1 x 10(8)/kg/apheresis.
- Collected progenitor cells correlated with WBC and MNC content in the apheresis product.
Conclusions:
- The modified Fenwal CS-3000 Plus with SVCC is effective for PBSC collection across three different mobilization regimens.
- This optimized apheresis protocol yields a small volume product with minimal platelets and high MNC content.
- The modified CS-3000 Plus is recommended for routine PBSC collection.