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Purification, characterization and partial sequencing of the heart fatty acid-binding protein from Bufo arenarum
1Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Abstract:
A 15.7 kDa fatty acid-binding protein from toad heart was purified by gel-filtration chromatography on Sephadex G-75 followed by anion-exchange chromatography on a Mono-Q column. Purity was confirmed by gel electrophoresis and isoelectric focusing. Molecular mass, isoelectric point, amino acid composition and partial internal sequence showed that the protein is related to mammalian heart fatty acid-binding proteins.