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Scrapie in vitro: agent replication and reduced cell yield

R I Carp, G S Merz, P C Licursi

    Infection and Immunity
    |July 1, 1976
    PubMed
    Summary
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    Scrapie infection in mice brain homogenates reduced mouse cell yield and altered immune cell percentages. The infectious agent replicated in cultured cells, causing scrapie disease and indicating a transmissible spongiform encephalopathy agent.

    Area of Science:

    • Neuroscience
    • Infectious Diseases
    • Cell Biology

    Background:

    • Scrapie is a transmissible spongiform encephalopathy affecting mice.
    • Understanding the replication and effects of the scrapie agent is crucial for disease research.

    Purpose of the Study:

    • To investigate the effects of scrapie infection on mouse cell cultures.
    • To determine if the scrapie agent replicates in cultured cells and retains infectivity.

    Main Methods:

    • Exposure of PAM cells to scrapie-infected mouse brain homogenates.
    • Assessing cell yield and immune cell profiles in treated mice.
    • Filtration to estimate the size of the infectious agent.

    Main Results:

    • Scrapie exposure reduced PAM cell yield from passage 2/3 to 18.

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  • Lysates from treated cells induced similar effects in fresh cultures.
  • Mice inoculated with lysates showed reduced neutrophils and developed scrapie disease.
  • The infectious agent was estimated to be 25-50 nm in diameter.

    • Conclusions:

    • The scrapie agent replicated in PAM cells, retaining its ability to induce disease.
    • PAM cells provide a model for studying scrapie agent replication and pathogenesis.
    • The study confirms the transmissible nature of the scrapie agent and its biological effects.