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Related Experiment Videos

SCID mouse spleen does not support scrapie agent replication

K I O'Rourke1, T P Huff, C W Leathers

  • 1United States Department of Agriculture, Animal Disease Research Unit, Pullman, Washington 99164-7030.

The Journal of General Virology
|June 1, 1994
PubMed
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Functional lymphocytes are crucial for scrapie agent infectivity and PrPSc accumulation in mouse spleens. Severe combined immunodeficiency (SCID) mice lacking these cells showed no detectable PrPSc, highlighting their importance in prion disease progression.

Area of Science:

  • Neuroimmunology
  • Prion Disease Research
  • Immunology

Background:

  • Scrapie is a transmissible spongiform encephalopathy affecting sheep and goats.
  • The accumulation of abnormal prion protein (PrPSc) is a hallmark of prion diseases.
  • The role of the immune system, particularly lymphocytes and follicular dendritic cells, in prion pathogenesis is not fully understood.

Purpose of the Study:

  • To investigate the role of functional lymphocytes and follicular dendritic cells in splenic infectivity and PrPSc accumulation.
  • To compare scrapie agent (strain ME7) progression in immunocompetent BALB/c mice and severe combined immunodeficiency (SCID) mice.

Main Methods:

  • Intracerebral and intraperitoneal inoculation of BALB/c and SCID mice with scrapie agent strain ME7.
  • Monitoring for clinical signs and microscopic lesions characteristic of scrapie.

Related Experiment Videos

  • Immunoblot analysis of spleen tissues to detect PrPSc accumulation.
  • Assessing splenic infectivity in SCID mice post-infection.

    • Main Results:

    • Both BALB/c and SCID mice inoculated intracerebrally developed clinical signs and lesions of scrapie.
    • PrPSc was detectable in spleens of terminally affected BALB/c mice but not in SCID mice.
    • SCID mouse spleens showed neither infectivity nor PrPSc within 90 days of intraperitoneal infection.

    Conclusions:

    • Functional lymphocytes and follicular dendritic cells are essential for PrPSc accumulation and splenic infectivity in scrapie.
    • The absence of these immune components in SCID mice prevents systemic prion spread and accumulation in the spleen.