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Related Experiment Videos

Bidirectional synaptic transmission in Necturus taste buds

D A Ewald1, S D Roper

  • 1Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|June 1, 1994
PubMed
Summary

Taste bud basal cells modulate taste receptor cell sensitivity. These basal cells release serotonin (5-HT), enhancing receptor potential propagation and increasing overall taste sensitivity.

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Area of Science:

  • Neuroscience
  • Sensory Biology
  • Cell Biology

Background:

  • Taste buds contain diverse cell types, including receptor cells and basal cells.
  • Interactions between these cells are crucial for taste perception.
  • The precise role of basal cells in modulating taste receptor cell function remains incompletely understood.

Purpose of the Study:

  • To investigate synaptic connections between taste receptor cells and basal cells.
  • To determine if basal cells can modulate taste receptor cell activity.
  • To identify potential signaling molecules involved in basal cell modulation.

Main Methods:

  • Intracellular recordings from taste cells in Necturus lingual epithelium slices.
  • Application of chemical stimuli (KCl, CaCl2) to elicit receptor potentials.

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  • Direct electrical depolarization of individual receptor and basal cells.
  • Application of serotonin (5-HT) to assess its modulatory effects.

    • Main Results:

    • Chemostimulation of receptor cells evoked postsynaptic responses in basal cells, indicating a forward synapse.
    • Depolarizing receptor cells evoked responses in basal cells, suggesting synaptic convergence.
    • Repetitive electrical stimulation of basal cells modulated receptor cells, increasing receptor potential amplitude and input resistance.
    • Bath application of serotonin mimicked the effects of basal cell stimulation, suggesting 5-HT as a mediator.
    • No retrograde synaptic responses were observed from basal cells to receptor cells with single stimuli.

    Conclusions:

    • Taste bud basal cells form chemical synapses with receptor cells.
    • Basal cells release serotonin (5-HT) onto adjacent taste receptor cells.
    • This 5-HT release enhances electrotonic propagation of receptor potentials, increasing taste receptor cell chemosensitivity.