Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method for processing individual oocytes and embryos for electron microscopy

C Nogués1, M Martí, M Boada

  • 1Departament Biologia Cellular i Fisiologia, Facultat Ciències, Universitat Autònoma de Barcelona, Bellaterra, Spain.

Journal of Microscopy
|April 1, 1994
PubMed
Summary

A novel method simplifies electron microscopy sample preparation using specialized cages for scanning and agarose embedding for transmission electron microscopy. This technique minimizes sample loss and reduces labor for researchers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic and serum biomarkers of NSAID hypersensitivity reactions.

Frontiers in pharmacology·2025
Same author

Sarcomas developed in patients with Lynch Syndrome are enriched in pleomorphic soft-tissue sarcomas and are sensitive to immunotherapy.

European journal of cancer (Oxford, England : 1990)·2025
Same author

Progress in the Treatment of Alzheimer's Disease Is Needed - Position Statement of European Alzheimer's Disease Consortium (EADC) Investigators.

The journal of prevention of Alzheimer's disease·2024
Same author

Links between gender norms and the intergenerational transmission of health information in parents carrying BRCA1/2 pathogenic variants.

Journal of genetic counseling·2023
Same author

Letter to the Editor: AMBAR: A Therapeutical Approach for Alzheimer's Disease Patients Regardless of Amyloid Status.

The journal of prevention of Alzheimer's disease·2023
Same author

Toxicity and behavioural effects of ocfentanil and 2-furanylfentanyl in zebrafish larvae and mice.

Neurotoxicology·2023

Area of Science:

  • Microscopy
  • Biological Sciences
  • Materials Science

Background:

  • Handling delicate biological specimens for electron microscopy can be challenging.
  • Traditional methods often involve time-consuming steps and risk sample loss.

Purpose of the Study:

  • To describe a simplified and efficient method for preparing individual specimens for both scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
  • To reduce labor, minimize sample loss, and decrease the need for extensive manipulation during sample processing.

Main Methods:

  • For SEM: Specimens are dehydrated within custom cages (TAAB capsules with plankton nets).
  • For TEM: Specimens are pre-embedded in agarose before further processing.
  • The method aims to streamline the workflow for electron microscopy sample preparation.

Related Experiment Videos

Main Results:

  • The described procedure significantly reduces the need for manual handling techniques like mouth pipetting.
  • It also decreases the reliance on dissecting microscope observations during preparation.
  • Crucially, the method leads to a substantial reduction in sample loss.

Conclusions:

  • This simple method offers an effective solution for preparing individual specimens for SEM and TEM.
  • It presents a less labor-intensive approach with improved sample preservation.
  • The technique enhances the overall efficiency of electron microscopy sample preparation workflows.