Related Experiment Videos
Detection of bcrabl fusion mRNA in samples of whole, unfractionated blood
1Department of Neoplastic Diseases, Hahnemann University, Philadelphia, PA 19102-1192.
Leukemia Research
|June 1, 1994
Abstract:
Using a new strategy, capture-RT-PCR, bcrabl fusion mRNA sequences were specifically and sensitively detected in samples of whole blood from leukemia patients with the Philadelphia chromosome. Sample processing required only mixing blood with the chaotropic salt, GuSCN, and mRNA was captured during a short incubation of prepared blood with an affinity membrane. Immobilized mRNA sequences were amplified without elution. No radioisotopes or Southern transfer were needed.