[Interaction of heparinized polymer materials with plasma proteins and thrombocytes]

The interaction of heparinized polymer materials with human plasma proteins (serum albumin (SA), fibrinogen (F), antithrombin III (AT-III)) and platelets was studied. The level of SA and F adsorption irreversibility was demonstrated to depend on the mode of heparin immobilization. There was a negative correlation between the parameters of protein adsorption and the level of adsorbed AT-III. The relationship of the count of platelets adhered to the surface of heparinized polymer materials, to the time of contact was shown to be extreme. There was a correlation between the count of adhesive platelets and the parameters of plasma protein and AT-III adsorption. Based on experimental evidence, a scheme was proposed, which describes the impact of protein adsorption processes on the anticoagulative activity of surface-bound heparin, It is concluded that AT-III plays a particular role as the major component of the adsorption layer that is responsible for the nature of interactions of polymer materials with blood at the protein and cellular levels.