Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

13C isotopomer analyses in intact tissue using [13C]homonuclear decoupling

A D Sherry¹, P Zhao, A Wiethoff

¹Department of Chemistry, University of Texas at Dallas.

Magnetic Resonance in Medicine

|April 1, 1994

Summary

This study introduces a new method using [13C]homonuclear decoupling to improve the analysis of the citric acid cycle in vivo. This technique enhances spectral resolution, enabling detailed metabolic studies in complex biological systems.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Interpretation of long-term survival data from MONARCH 3. Letter to the Editor regarding "Abemaciclib plus a nonsteroidal aromatase inhibitor as initial therapy for HR+, HER2- advanced breast cancer: final overall survival results of MONARCH 3" by M. P. Goetz et al.

Annals of oncology : official journal of the European Society for Medical Oncology·2024

Same author

Using micro-synchrotron radiation x-ray fluorescence (µ-SRXRF) for trace metal imaging in the development of MRI contrast agents for prostate cancer imaging.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)·2022

Same author

Operation and performance of the ATLAS Tile Calorimeter in Run 1.

The European physical journal. C, Particles and fields·2019

Same author

Measurement of the azimuthal anisotropy of charged particles produced in <math> </math> = 5.02 TeV Pb+Pb collisions with the ATLAS detector.

The European physical journal. C, Particles and fields·2019

Same author

Search for Higgs boson pair production in the <math></math> channel using <math></math> collision data recorded at <math></math> <math></math> TeV with the ATLAS detector.

The European physical journal. C, Particles and fields·2019

Same author

Effects of deuteration on transamination and oxidation of hyperpolarized <sup>13</sup>C-Pyruvate in the isolated heart.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2019

Area of Science:

Biochemistry
Metabolic Engineering
Nuclear Magnetic Resonance Spectroscopy

Background:

Citric acid cycle metabolism is crucial for cellular energy production.
13C-enriched acetyl-CoA tracing reveals metabolic flux but is limited by spectral resolution.
Previous isotopomer analysis of glutamate 13C multiplets quantified substrate utilization and anaplerotic flux.

Purpose of the Study:

To overcome the spectral resolution limitations of in vivo 13C isotopomer analysis.
To develop a method for detailed metabolic studies in intact tissues.
To enable the extension of 13C isotopomer methods to complex in vivo metabolic conditions.

Main Methods:

Utilized [13C]homonuclear decoupling of the glutamate C3 resonance.
Collapsed complex nine-line resonances into simpler three-line multiplets.

Related Experiment Videos

Applied the method to isolated, perfused rat hearts for in vivo validation.

Main Results:

Achieved significant improvement in spectral resolution of 13C multiplets.
Demonstrated well-resolved three-line 13C multiplets in intact heart tissue.
Presented steady-state equations for isotopomer analysis from in vivo data.

Conclusions:

[13C]homonuclear decoupling effectively enhances resolution for 13C isotopomer analysis.
This technique allows for robust metabolic flux quantification in intact tissues.
The advancement opens new possibilities for studying complex in vivo metabolic states.