Changes in translational accuracy of Escherichia coli when folate metabolism is perturbed

Translational accuracy was monitored in Escherichia coli mutants which contain abnormal folate pools. A decrease in translational accuracy was indicated by the increased production of a T4 phage mutant containing a UGA mutation in a tail fibre gene. An E. coli folC mutant suppressed the phage mutant under conditions where it presumably accumulated methyl-tetrahydrofolate (methyl-THF). A UGA suppressor strain with the mutation affecting the primary structure of the tRNA(trp) normally suppressed the phage mutant. When the strain was made thymine-requiring it no longer suppressed. The accumulation of methyl-THF which permits suppression by thymine-requiring strains may act to interfere with suppression by the tRNA suppressor, possibly by changing the modification pattern of the tRNA.