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Prophage map of converting corynebacteriophage beta

W Laird, N Groman

    Journal of Virology
    |July 1, 1976
    PubMed
    Summary

    Researchers mapped corynebacteriophage beta, revealing a cyclic permutation between its prophage and vegetative forms. This finding supports the Campbell model and suggests toxin conversion by phages may stem from specialized transduction.

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    Proceedings of the National Academy of Sciences of the United States of America·1987

    Area of Science:

    • Bacteriophage genetics
    • Molecular biology
    • Microbial genetics

    Background:

    • Bacteriophage beta is a temperate phage that integrates into the Corynebacterium host genome.
    • Understanding phage integration and gene order is crucial for deciphering phage biology and host interactions.
    • Previous mapping efforts have focused on vegetative phage genomes, but prophage configurations require distinct approaches.

    Purpose of the Study:

    • To construct a genetic map of the corynebacteriophage beta prophage.
    • To compare the prophage map with the existing vegetative map of the phage.
    • To investigate the implications of the map comparison for phage integration models and evolution.

    Main Methods:

    • Utilized a system of unstable heteroimmune double lysogens to generate monolysogenic segregants.
    • Characterized recombinant phages derived from these segregants to establish the prophage map.
    • Compared the derived prophage map with the established vegetative map of corynebacteriophage beta.

    Main Results:

    • A seven-marker prophage map for corynebacteriophage beta was successfully constructed.
    • The prophage map was found to be a cyclic permutation of the vegetative map.
    • The gene for toxin was located at one end and the gene for phage immunity at the other end of the prophage map.

    Conclusions:

    • The observed cyclic permutation supports the Campbell model for phage integration, specifically lambda phage, with a designated attachment site between toxin and immunity genes.
    • The prophage map's gene order suggests that toxin-converting phages may have evolved from specialized transducing phages targeting the toxin gene.
    • This study provides novel insights into the genetic organization of temperate phages in their integrated state and their evolutionary origins.

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