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Development of a phage typing system for Staphylococcus hyicus

¹Department of Microbiology, National Veterinary Laboratory, Copenhagen.

Research in Microbiology

|March 1, 1993

Summary

A new phage typing system effectively identifies Staphylococcus hyicus strains, aiding in diagnosing pig exudative epidermidis and supporting epidemiological studies.

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Area of Science:

Veterinary Microbiology
Bacteriology
Virology

Background:

Staphylococcus hyicus is a significant swine pathogen.
Accurate identification and epidemiological tracking of S. hyicus are crucial for disease control.

Purpose of the Study:

To develop and evaluate a bacteriophage typing system for Staphylococcus hyicus.
To assess the utility of this system in diagnosing and studying S. hyicus infections in pigs.

Main Methods:

Bacteriophages were induced from Staphylococcus hyicus strains using mitomycin C.
A set of 23 phages with distinct lytic spectra was established for phage typing.
The system's typability, reproducibility, and discriminatory power were assessed using various S. hyicus isolates.

Main Results:

Phages were successfully isolated from 98% of S. hyicus strains.
The developed phage typing system achieved 92% typability for Danish porcine S. hyicus strains, yielding 16 distinct phage types.
High reproducibility (96%) was observed, with typability varying for international porcine strains (52-80%).
Phage type A/B/C/W was significantly associated with piglets exhibiting exudative epidermidis.

Conclusions:

The developed bacteriophage typing system is a valuable tool for the diagnosis of exudative epidermidis in pigs.
This system holds potential for epidemiological investigations of Staphylococcus hyicus.

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