Related Experiment Video
Updated: Aug 3, 2026

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Published on: April 16, 2012
H-2-specific T suppressor cells. I. Evidence for T suppressor inducer and effector molecules in suppression
A Malley1, M Zeleny-Pooley, S Kelleher-Mayo
1Oregon Regional Primate Research Center, Beaverton 97006-3499.
Abstract:
The induction of H-2-specific Ts cells was accomplished by the i.v. injection of X-irradiated (2000R) C57BL/6 spleen cells into CBA/J mice. These Ts cells significantly (78%) suppressed the delayed type hypersensitivity (DTH) response in CBA/J mice injected s.c. with X-irradiated (2000R) C57BL/6 spleen cells and given a footpad challenge with the same cell population 5 days later. We have shown that both CD4 and CD8 T cells were involved in the observed suppression, and these cells secrete T suppressor inducer (Tsfi) and T suppressor effector (Tsfe) molecules. Both Tsfi and Tsfe molecules were shown to significantly inhibit (> 87%) one-way mixed lymphocyte responses between CBA/J and mitomycin C-treated C57BL/6 spleen cells. Using an adoptive transfer method, we showed that mice given both a primary and secondary immunization with X-irradiated C57BL/6 spleen cells to induce H-2-specific Ts cells contain a significantly greater number of Ts cells than mice given only a primary immunization, suggesting the presence of memory Ts cells.
More Related Videos
08:20In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
Published on: December 30, 2016
18:48In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
Published on: August 12, 2017
Related Concept Videos
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cytotoxic T Cells-mediated Immune Response
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Tumor Immunotherapy