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ICAM-3 expression on endothelium in lymphoid malignancy
I Doussis-Anagnostopoulou1, L Kaklamanis, J Cordell
1Nuffield Department of Pathology, John Radcliffe Hospital, Oxford, United Kingdom.
The American Journal of Pathology
|October 1, 1993
Summary
Intercellular adhesion molecule-3 (ICAM-3) is found on most lymphoid cells but not on Reed-Sternberg cells in Hodgkin's disease. Endothelial ICAM-3 expression may be induced in lymphoid neoplasms.
Area of Science:
- Immunology
- Cell Biology
- Oncology
Background:
- Intercellular adhesion molecule-3 (ICAM-3) is a recently characterized immunoglobulin superfamily member and a receptor for lymphocyte function-associated antigen molecule-1.
- ICAM-3 plays a role in immune cell interactions.
Purpose of the Study:
- To investigate the expression pattern of ICAM-3 in reactive lymph nodes, Hodgkin's disease, and non-Hodgkin's lymphomas.
- To determine if ICAM-3 is expressed on malignant cells or associated endothelial cells in these conditions.
Main Methods:
- Immunocytochemical analysis using specific monoclonal antibodies.
- Examination of tissue samples from reactive lymph nodes, Hodgkin's disease, and non-Hodgkin's lymphomas.
Main Results:
- ICAM-3 was detected on T and B cells in mantle zones and some follicular center cells in reactive lymph nodes.
- In Hodgkin's disease, ICAM-3 was absent from Reed-Sternberg cells but present on reactive lymphoid cells and frequently on endothelial cells.
- Similar endothelial ICAM-3 expression was observed in a subset of non-Hodgkin's lymphomas.
Conclusions:
- ICAM-3 expression is differential in lymphoid neoplasms, being absent from malignant cells in Hodgkin's disease.
- Endothelial ICAM-3 expression can be induced in lymphoid neoplasms, potentially via cytokine-mediated mechanisms, similar to ICAM-1 and ICAM-2.