Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

White pulp reconstitution after human bone marrow transplantation

A Nakayama1, N Hirabayashi, M Ito

  • 1First Department of Pathology, Nagoya University School of Medicine, Japan.

The American Journal of Pathology
|October 1, 1993
PubMed
Summary

The spleen's white pulp reconstitutes sequentially after bone marrow transplantation (BMT), starting with T cell areas, then B cell follicles, and lastly marginal zones. Delayed marginal zone recovery impacts immune function in BMT survivors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutrophil to lymphocyte ratio and tumour burden for treatment efficacy stratification in renal cell carcinoma patients receiving nivolumab plus ipilimumab.

ESMO real world data and digital oncology·2026
Same author

Genomic and transcriptomic analyses of melanoma in Japanese patients reveal candidate biomarkers for immune checkpoint inhibitor responders.

Communications medicine·2026
Same author

Gastrointestinal: Relapsed Type 1 autoimmune pancreatitis mimicking pancreatic cancer.

Journal of gastroenterology and hepatology·2024
Same author

Gastrointestinal: Successful endoscopic treatment of multiple large common bile duct stones with benign biliary stricture in a case of distal gastrectomy with Billroth II reconstruction.

Journal of gastroenterology and hepatology·2022
Same author

Gastrointestinal: A case of duodenal cancer with subepithelial lesion-like morphology.

Journal of gastroenterology and hepatology·2021
Same author

A detailed analysis of the spin-crossover reaction of H<sub>2</sub>S binding to heme and the six-coordinated FeP(Im)-HS<sup>-</sup> porphyrin complex.

Journal of inorganic biochemistry·2020

Area of Science:

  • Immunology
  • Hematology
  • Transplantation Biology

Background:

  • Bone marrow transplantation (BMT) is a critical therapy for hematologic diseases.
  • Understanding immune reconstitution post-BMT is vital for patient recovery and long-term health.
  • The spleen's white pulp plays a key role in immune surveillance and response.

Purpose of the Study:

  • To elucidate the sequential process of white pulp reconstitution in the spleen following BMT.
  • To correlate the morphological recovery of white pulp components with immune cell populations.
  • To identify potential causes for impaired immune function in long-term survivors.

Main Methods:

  • Histopathological and immunohistochemical analysis of spleen tissues from 24 BMT recipients.
  • Analysis of spleens from recipients with survival times ranging from 34 to 303 days post-BMT.

Related Experiment Videos

  • Evaluation of T cells, B cells, and dendritic cell populations within the white pulp.

    • Main Results:

    • White pulp was atrophic up to 3 months post-BMT, primarily composed of T cells in periarteriolar lymphatic sheaths (PALS).
    • B cell aggregation into primary follicles occurred around 100 days, but marginal zones were absent.
    • Full white pulp structure (PALS, follicles, marginal zones) was observed in most recipients after 4 months, with poor marginal zone recovery up to 10 months.

    Conclusions:

    • White pulp reconstitution post-BMT is a sequential process: PALS, then lymphoid follicles, and finally the marginal zone.
    • The development of PALS and lymphoid follicles correlates with interdigitating and follicular dendritic cells, respectively.
    • Delayed marginal zone reconstitution is implicated in the functional asplenia observed in long-term BMT survivors.