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Toxin production by Pasteurella multocida isolated from rabbits with atrophic rhinitis
R F DiGiacomo1, B J Deeb, S J Brodie
1Department of Comparative Medicine, School of Medicine, University of Washington, Seattle 98195.
American Journal of Veterinary Research
|August 1, 1993
Summary
Pasteurella multocida infection causes turbinate atrophy in rabbits. While toxin production was investigated, it was only detected in vitro, not in vivo, suggesting complex mechanisms in atrophic rhinitis.
Area of Science:
- Veterinary Pathology
- Microbiology
- Animal Disease Research
Background:
- Naturally acquired turbinate atrophy in rabbits is linked to Pasteurella multocida.
- Atrophic rhinitis causes significant turbinate bone damage in affected animals.
Purpose of the Study:
- To investigate toxin production by Pasteurella multocida isolates.
- To determine the relationship between P. multocida toxins and the development of atrophic rhinitis in rabbits.
Main Methods:
- Ten P. multocida serotype A:12 isolates were obtained from rabbits with naturally occurring atrophic rhinitis.
- Specific-pathogen-free rabbits were inoculated intranasally with P. multocida isolates or bacterial filtrates.
- In vitro assays included cytotoxicity tests on bovine embryonic turbinate cells and ELISA.
Main Results:
- Intranasal inoculation with P. multocida isolates successfully reproduced rhinitis and turbinate atrophy in rabbits.
- Inoculation with bacterial filtrates did not induce turbinate atrophy.
- Cytotoxicity was detected in vitro using cell culture assays with bacterial extracts, but not in other in vitro tests.
Conclusions:
- Pasteurella multocida isolates can experimentally induce turbinate atrophy in rabbits.
- The toxin responsible for turbinate atrophy was detected in vitro but not confirmed in vivo, indicating a complex pathogenesis.