Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ferredoxin biosynthesis in Euglena gracilis

R S Matson, T Kimura

    Biochimica Et Biophysica Acta
    |August 2, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Ferredoxin in Euglena gracilis is synthesized using cytoplasmic ribosomes, not chloroplast ones. This protein is produced from nuclear DNA, even in mutants lacking chloroplasts.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Fiberoptic DNA sensor array capable of detecting point mutations.

    Analytical biochemistry·1997
    Same author

    Biopolymer synthesis on polypropylene supports: oligonucleotide arrays.

    Analytical biochemistry·1995
    Same author

    A rapid scanning strip for tri- and dinucleotide short tandem repeats.

    Nucleic acids research·1994
    Same author

    Biopolymer synthesis on polypropylene supports. I. Oligonucleotides.

    Analytical biochemistry·1994
    Same author

    Effects of pirenzepine and hexamethonium on adrenal catecholamine release in responses to endogenous and exogenous acetylcholine in anesthetized dogs.

    Journal of cardiovascular pharmacology·1992
    Same author

    Autoimmunomodulation. Age-related opioid differences in vertebrate and invertebrate immune systems.

    Annals of the New York Academy of Sciences·1992
    Same journal

    Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Molecular Basis of Disease Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    General Subjects Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

    Biochimica et biophysica acta·2019
    Same journal

    Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

    Biochimica et biophysica acta·2019
    Same journal

    Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

    Biochimica et biophysica acta·2019
    See all related articles

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Ferredoxins are crucial non-heme iron proteins involved in electron transfer.
    • Understanding ferredoxin synthesis in eukaryotic organisms like Euglena gracilis is vital for comprehending cellular processes.
    • Euglena gracilis offers a unique model for studying organelle-specific gene expression and protein synthesis.

    Purpose of the Study:

    • To determine the site of ferredoxin synthesis in Euglena gracilis.
    • To investigate the origin of ferredoxin's transcript.
    • To elucidate the role of cytoplasmic versus chloroplast ribosomes in ferredoxin production.

    Main Methods:

    • Utilizing selective antibiotic inhibitors of protein synthesis (cycloheximide, chloramphenicol, erythromycin) to block translation.

    Related Experiment Videos

  • Analyzing ferredoxin content in wild-type and streptomycin-bleached Euglena gracilis mutants.
  • Employing rifampicin to inhibit chloroplast DNA-dependent RNA polymerase.

    • Main Results:

    • Ferredoxin synthesis was completely inhibited by cycloheximide, an inhibitor of cytoplasmic (80s-type) ribosomes.
    • Inhibitors of chloroplast (70s-type) translation (chloramphenicol, erythromycin) did not affect ferredoxin synthesis.
    • Ferredoxin was detected in a streptomycin-bleached mutant lacking chloroplast DNA, and its synthesis was unaffected by rifampicin.

    Conclusions:

    • Ferredoxin in Euglena gracilis is synthesized on cytoplasmic ribosomes.
    • The ferredoxin transcript originates from the nuclear genome, not chloroplast DNA.
    • These findings highlight the extranuclear synthesis of ferredoxin in Euglena gracilis.