PCR cloning and sequence of the murine GPIIb gene promoter

The promoter region of the murine GPIIb gene was cloned by PCR using oligonucleotides corresponding to the human gene promoter. Analysis of the sequence revealed homologies among species and the conservation of DNA motifs which could be implicated in the regulation of the gene. Transfection experiments show that the murine promoter contains informations for a tissue specific expression of the gene in megakaryocytic cell lines.