Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acyl structure regulates galactosylceramide's interfacial interactions

S Ali1, J M Smaby, R E Brown

  • 1Hormel Institute, University of Minnesota, Austin 55912.

Biochemistry
|November 2, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of propofol in patients with food allergies.

Anaesthesia·2008
Same author

KiSS-1 mRNA in adipose tissue is regulated by sex hormones and food intake.

Molecular and cellular endocrinology·2007
Same author

Which port in a storm?

Anaesthesia·2007
Same author

Electrophysiological characterization of neurons in the dorsolateral pontine rapid-eye-movement sleep induction zone of the rat: Intrinsic membrane properties and responses to carbachol and orexins.

Neuroscience·2006
Same author

Visual detection, pattern discrimination and visual acuity in 14 strains of mice.

Genes, brain, and behavior·2006
Same author

Effects of arousal- and feeding-related neuropeptides on dopaminergic and GABAergic neurons in the ventral tegmental area of the rat.

The European journal of neuroscience·2006
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
Same journal

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
See all related articles

Synthesized galactosylceramides (GalCer) reveal how acyl chain saturation and length influence their surface behavior. Unsaturated acyl chains promote liquid-expanded films, while saturated chains form condensed films, impacting surface potential.

Area of Science:

  • Biochemistry
  • Surface Chemistry
  • Lipid Research

Background:

  • Galactosylceramides (GalCer) are crucial glycosphingolipids with diverse biological roles.
  • The physical properties of GalCer at interfaces are influenced by their acyl chain composition.
  • Understanding GalCer behavior is vital for elucidating membrane structure and function.

Purpose of the Study:

  • To synthesize and characterize galactosylceramides (GalCer) with defined acyl chain structures.
  • To investigate the impact of acyl chain saturation and length on GalCer's interfacial behavior.
  • To determine the role of acyl unsaturation in regulating GalCer's surface properties.

Main Methods:

  • Synthesis of homogeneous galactosylceramides (GalCer) with varying acyl chain saturation (0, 1, or 2 cis double bonds).

Related Experiment Videos

  • Characterization at an argon-aqueous buffer interface using Langmuir film balance.
  • Measurement of surface pressure and surface potential as a function of molecular area.

    • Main Results:

    • Saturated acyl chains (e.g., N-24:0-GalSph, N-18:0-GalSph, N-16:0-GalSph) formed condensed films, similar to bovine brain GalCer.
    • GalCer derivatives with mono-unsaturated acyl chains (N-24:1 delta 15-GalSph, N-22:1 delta 13-GalSph) exhibited phase transitions.
    • Short saturated or long di-unsaturated acyl chains resulted in liquid-expanded GalCer films; surface potentials varied with acyl chain saturation.

    Conclusions:

    • Acyl chain saturation significantly modulates the surface behavior of galactosylceramides (GalCer).
    • Unsaturation in acyl chains promotes more fluid (liquid-expanded) film formation.
    • Saturated acyl chains lead to more rigid (condensed) films with altered surface potentials.