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Related Experiment Videos

cDNA sequence for mouse caltractin

K Ogawa1, T Shimizu

  • 1Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.

Biochimica Et Biophysica Acta
|October 19, 1993
PubMed
Summary

Researchers discovered mouse caltractin, a contractile calcium-binding protein, in the testis. This protein, previously only found in algae, has a similar molecular mass to its algal counterpart.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Caltractin is a contractile calcium-binding protein.
  • Caltractin has been exclusively identified in algae species until now.

Purpose of the Study:

  • To report the first isolation and sequencing of mouse caltractin.
  • To investigate the presence of caltractin in mammalian species.

Main Methods:

  • Immunoscreening of a mouse testis cDNA library.
  • Isolation and sequencing of cDNA encoding mouse caltractin.

Main Results:

  • Mouse caltractin cDNA was successfully isolated and sequenced.
  • Mouse caltractin has a molecular mass of 20 kDa.
  • This marks the first identification of caltractin in a non-algal species.

Conclusions:

  • Caltractin is present in mammals, specifically in mouse testis.
  • The molecular characteristics of mouse caltractin are conserved with algal caltractin.

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