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DNA binding factor GT-2 from Arabidopsis

R M Kuhn1, T Caspar, K Dehesh

  • 1Department of Plant Biology, University of California-Berkeley.

Plant Molecular Biology
|October 1, 1993
PubMed
Summary

Researchers identified a conserved DNA-binding factor, Arabidopsis GT-2, in plants. This protein shows similar DNA-binding characteristics to rice GT-2, suggesting evolutionary conservation between monocots and dicots.

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Area of Science:

  • Plant Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA-binding factors regulate gene expression by interacting with specific DNA sequences.
  • The GT-box motif is found in the promoter regions of various plant genes.
  • Previous studies identified GT-2 in rice and GT-1a/B2F in tobacco.

Purpose of the Study:

  • To isolate and characterize a DNA-binding factor from Arabidopsis homologous to rice GT-2.
  • To investigate the structural and functional conservation of this factor between different plant species.

Main Methods:

  • DNA hybridization using a rice GT-2 factor cDNA clone.
  • Sequencing of complementary DNA (cDNA) clones.
  • Bioinformatic analysis of predicted protein structure and homology.
  • DNA-binding assays to compare binding characteristics.

Main Results:

  • Arabidopsis GT-2 gene is a single copy, transcribed into a 2.1 kb mRNA.
  • The predicted 65 kDa protein shares significant homology with rice GT-2, including conserved trihelix structures.
  • Arabidopsis GT-2 exhibits similar DNA-binding properties to rice GT-2, but differs from tobacco GT-1a/B2F.
  • Limited homology was found with other known DNA-binding factors.

Conclusions:

  • A DNA-binding factor with conserved structural domains and target-sequence specificity exists between monocots (rice) and dicots (Arabidopsis).
  • The Arabidopsis GT-2 factor plays a role in regulating gene expression through GT-box motifs.
  • This finding highlights the evolutionary conservation of gene regulatory mechanisms in plants.

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