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A cDNA clone for a pathogenesis-related protein 1 from barley
A Muradov1, L Petrasovits, A Davidson
1Department of Biochemistry, University of Queensland, Brisbane, Australia.
Plant Molecular Biology
|October 1, 1993
Summary
Researchers identified a barley gene (PRb-1) that activates upon powdery mildew infection in resistant plants. This gene
Area of Science:
- Plant Pathology
- Molecular Biology
- Biochemistry
Background:
- Powdery mildew is a significant disease affecting barley production worldwide.
- Plant resistance mechanisms involve the activation of specific genes and proteins.
- Pathogenesis-Related (PR) proteins are known to play a role in plant defense responses.
Purpose of the Study:
- To isolate and characterize a gene involved in barley's defense against powdery mildew.
- To investigate the expression patterns of this gene in response to pathogen infection and chemical treatments.
Main Methods:
- Isolation and characterization of a barley cDNA clone (PRb-1).
- Deduction of amino acid sequence and homology analysis.
- Northern blot analysis to detect mRNA accumulation.
- Gene expression analysis following inoculation and treatment with plant hormones and signaling molecules.
Main Results:
- A novel barley gene, PRb-1, was identified, encoding a 15 kDa mature peptide with homology to PR-1-like proteins.
- PRb-1 mRNA accumulated significantly in resistant barley 12 hours post-inoculation with Erysiphe graminis.
- PRb-1 gene expression was also induced by ethylene, salicylic acid, methyl jasmonate, and 2,6-dichloro-isonicotinic acid in resistant plants.
Conclusions:
- PRb-1 is a pathogenesis-related gene in barley, likely involved in the plant's defense against powdery mildew.
- Its induction by various signaling molecules suggests a role in the broader plant immune signaling network.
- Understanding PRb-1 function can contribute to developing disease-resistant barley cultivars.