Related Experiment Videos
Interplay between differentiation and cell cycle arrest at G1 in phorbol diester-treated human myelocytic leukemia
1Life Science Group, Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
International Journal of Hematology
|August 1, 1993
Abstract:
The cell cycle arrest at G1 of myelocytic leukemia cells by incubation with phorbol diesters was examined in HL-60 cells and more mature THP-1 cells. We found: (1) induction of phenotypic parameters of differentiation prior to G1 arrest; (2) a long induction period in HL-60 cells compared to zero in THP-1 cells; and (3) strong inhibition of G1 arrest with a protein kinase C inhibitor in THP-1 cells. We conclude, from these lines of evidence and some additional information, that differentiation induces G1 arrest. At present, the most plausible causative molecule is Mac-1.