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Evidence for extrachromosomal elements in Lactobacillus

B M Chassy, E Gibson, A Giuffrida

    Journal of Bacteriology
    |September 1, 1976
    PubMed
    Summary
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    Researchers discovered plasmids in three lactobacilli strains, including Lactobacillus casei subsp. casei 64H and L. casei subsp. rhamnosus OC91. This marks the first evidence of plasmids in lactobacilli, though their function remains unknown.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Lactobacilli are a group of bacteria commonly found in the human gut and are known for their probiotic properties.
    • The genetic makeup of many bacterial species includes plasmids, which are extrachromosomal DNA molecules that can confer advantageous traits.
    • Previous research had not definitively identified plasmids within lactobacilli strains.

    Purpose of the Study:

    • To investigate the presence of plasmids in specific strains of lactobacilli.
    • To characterize the molecular weight of any identified plasmids.
    • To establish the foundational evidence for plasmids in lactobacilli.

    Main Methods:

    • Plasmid DNA was extracted from three selected lactobacilli strains: Lactobacillus casei subsp. casei 64H, L. casei subsp. rhamnosus OC91, and L. coryniformis M34.

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  • Extracted DNA was analyzed using gel electrophoresis to detect and size plasmids.
  • Molecular weights were estimated based on comparison with known DNA markers.

    • Main Results:

    • Plasmids were detected in two of the three examined lactobacilli strains.
    • A plasmid with a molecular weight of 23 x 10^6 Daltons was identified in Lactobacillus casei subsp. casei 64H.
    • A plasmid with a molecular weight of 16 x 10^6 Daltons was identified in L. casei subsp. rhamnosus OC91.

    Conclusions:

    • This study provides the first documented evidence of plasmid presence in lactobacilli.
    • The identified plasmids in L. casei subsp. casei 64H and L. casei subsp. rhamnosus OC91 represent novel findings.
    • The functional significance of these plasmids in lactobacilli remains to be elucidated in future research.