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Hemagglutination by Pasteurellaceae isolated from rodents
1Department of Laboratory Animal Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|June 1, 1993
Summary
Pasteurellaceae bacteria from mice and Mastomys showed hemagglutinating properties, suggesting a potential relationship and distinctness from other rodent Pasteurellaceae. This study explored bacterial adhesion mechanisms in rodent hosts.
Area of Science:
- Microbiology
- Bacterial Pathogenesis
- Host-Microbe Interactions
Background:
- Pasteurellaceae, particularly P. pneumotropica, are linked to severe rodent respiratory disease outbreaks.
- Host-specific parasitism and tropism of Pasteurellaceae in rodents remain understudied.
- Bacterial adhesion mechanisms, such as hemagglutination, are crucial for host tropism.
Purpose of the Study:
- To investigate the hemagglutinating (HA) properties of Pasteurellaceae isolates from various rodent species.
- To understand the potential role of bacterial surface structures in host specificity.
- To explore relationships between Pasteurellaceae from different rodent hosts.
Main Methods:
- Collected 44 Pasteurellaceae isolates from mice, rats, hamsters, gerbils, and Mastomys.
- Assessed hemagglutinating activity using human (O Rh+) and canine red blood cells (RBCs).
- Utilized inhibition assays (sugars, heat, glutaraldehyde, NaIO4) and electron microscopy.
Main Results:
- Only mouse and Mastomys isolates exhibited hemagglutination against human and canine RBCs.
- Hemagglutination was sensitive to heat and NaIO4, indicating involvement of polysaccharide structures.
- Electron microscopy revealed fimbriae in all isolates, but a regularly structured (RS) layer was present only in some HA+ isolates.
Conclusions:
- Pasteurellaceae from mice and Mastomys may share relatedness and differ from isolates of other rodent species.
- Bacterial surface structures, potentially including an RS layer, may play a role in host tropism.
- Further research into Pasteurellaceae adhesion mechanisms is warranted for understanding rodent diseases.