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Related Experiment Videos

Activated partial thromboplastin time reagents: an evaluation

S Howarth1

  • 1Department of Pathology, King Edward Memorial Hospital for Women, Subiaco, Perth, Western Australia.

British Journal of Biomedical Science
|June 1, 1993
PubMed
Summary
This summary is machine-generated.

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This study evaluated nine automated activated partial thromboplastin time (APTT) reagents. Only one reagent met all criteria for replacing manual reagents in laboratory diagnostics.

Area of Science:

  • Clinical Chemistry
  • Hematology
  • Diagnostic Reagent Evaluation

Background:

  • The activated partial thromboplastin time (APTT) test is crucial for monitoring heparin therapy and diagnosing coagulation disorders.
  • Evaluating automated APTT reagents is essential for ensuring reliable laboratory diagnostics and efficient workflow.

Purpose of the Study:

  • To assess the performance of nine automated activated partial thromboplastin time (APTT) reagents against a manual method.
  • To identify a suitable automated APTT reagent that meets specific stability, heparin sensitivity, factor deficiency, and lupus anticoagulant detection criteria.

Main Methods:

  • Nine automated APTT reagents were tested on an automated coagulometer.
  • Reagents were evaluated for working stability (4-8°C for 7 days) and heparin sensitivity (0-0.8 IU/ml).

Related Experiment Videos

  • Sensitivity to factor deficiencies (VIII, IX, XI, XII) and lupus anticoagulants was assessed using standardized and deficient plasmas.

    • Main Results:

    • Only one of the nine evaluated automated APTT reagents met all predefined performance criteria.
    • This single reagent demonstrated acceptable working stability, heparin sensitivity, and specificity for factor deficiencies and lupus anticoagulants.

    Conclusions:

    • A single automated activated partial thromboplastin time (APTT) reagent was identified as a viable replacement for manual methods.
    • The selection process ensures diagnostic accuracy and reliability in coagulation testing.