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Ambroxol inhibits endotoxin-induced lipid peroxidation in mice
D Nowak1, T Pietras, A Antczak
1Department of Pneumonology and Allergology, Medical Academy, Lódź, Poland.
Abstract:
Administration of ambroxol (70 mg/kg ip) once a day for 3 days protected lung and heart lipids from lipopolysaccharide (LPS, 17 mg/kg)-induced oxidative stress in mice. Ambroxol as a lipid peroxidation inhibitor was almost as active as an equivalent dose of N-acetylcysteine (27.6 mg/kg), a well known antioxidant. The lung and heart levels of conjugated dienes in animals pretreated with ambroxol were 3.3- and 1.7-times lower (p < 0.05 and p < 0.01) than those observed in the control group which received only buffer and subsequently LPS. These results indicate that ambroxol can sufficiently inhibit the harmful process of lipid peroxidation in vivo.
Insights
Ambroxol protects against oxidative stress in mouse lungs and hearts by inhibiting lipid peroxidation. This antioxidant effect is comparable to N-acetylcysteine, suggesting ambroxol
Area of Science:
- Biochemistry
- Pharmacology
- Toxicology
Background:
- Oxidative stress, induced by lipopolysaccharide (LPS), damages lipids in vital organs.
- Lipid peroxidation is a key mechanism in LPS-induced cellular damage.
- Antioxidants play a crucial role in mitigating oxidative stress.
Purpose of the Study:
- To investigate the efficacy of ambroxol as a lipid peroxidation inhibitor in vivo.
- To compare ambroxol's antioxidant activity with N-acetylcysteine.
- To assess the protective effects of ambroxol against LPS-induced oxidative stress in mouse lungs and hearts.
Main Methods:
- Mice were administered ambroxol (70 mg/kg) or N-acetylcysteine (27.6 mg/kg) daily for 3 days.
- Lipopolysaccharide (LPS) was administered to induce oxidative stress.
- Levels of conjugated dienes, markers of lipid peroxidation, were measured in lung and heart tissues.
Main Results:
- Ambroxol pretreatment significantly reduced lipid peroxidation in mouse lungs (3.3-fold lower) and hearts (1.7-fold lower) compared to controls.
- The lipid peroxidation inhibitory activity of ambroxol was comparable to N-acetylcysteine.
- Significant reduction in conjugated dienes was observed (p < 0.05 and p < 0.01).
Conclusions:
- Ambroxol demonstrates potent in vivo inhibition of lipid peroxidation.
- Ambroxol offers significant protection against LPS-induced oxidative stress in lung and heart tissues.
- Ambroxol is a potential therapeutic agent for conditions involving oxidative damage.