Related Experiment Video
Updated: Aug 13, 2026
07:29
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Lipid peroxidation and its role in atherosclerosis
1Institute of Biochemistry, University of Graz, Austria.
British Medical Bulletin
|July 1, 1993
Summary
Oxidative modification of low-density lipoprotein (LDL) is key in atherosclerosis. Enhancing antioxidant status, particularly with alpha-tocopherol, may prevent this disease.
Area of Science:
- Biochemistry
- Pathology
- Cardiovascular Science
Background:
- Atherosclerosis pathogenesis involves low-density lipoprotein (LDL) oxidation.
- LDL oxidation is a free radical-driven lipid peroxidation process.
- Aldehydic breakdown products modify LDL apoprotein, impacting its function.
Purpose of the Study:
- To elucidate the role of LDL oxidation in atherosclerosis.
- To investigate the mechanism of aldehyde modification of apoB protein.
- To explore the potential of antioxidant status in preventing atherosclerosis.
Main Methods:
- Analysis of lipid peroxidation pathways in LDL.
- Characterization of aldehyde modification of apoB protein.
- Assessment of scavenger receptor-mediated uptake of modified LDL.
Main Results:
- Aldehyde-modified apoB exhibits altered receptor affinity.
- Uncontrolled scavenger uptake leads to foam cell formation and atherosclerotic lesion initiation.
- Endogenous antioxidants, notably alpha-tocopherol, can prevent LDL oxidation.
Conclusions:
- Oxidative modification of LDL is a critical step in atherosclerosis development.
- Aldehydic products of lipid peroxidation contribute to lesion progression.
- Improving antioxidant status presents a potential strategy for atherosclerosis prevention.
Related Concept Videos
01:38Inflammation
Overview
01:24Peroxisomes
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
01:20Radical Autoxidation
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
01:26Coronary Artery Disease II: Pathophysiology
Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
01:30Atherosclerosis I: Introduction
Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
01:30Peripheral Artery Disease I: Introduction
Peripheral artery disease (PAD) predominantly results from atherosclerosis, which involves the accumulation of fatty deposits, or plaques, within the walls of arteries. This causes them to narrow and harden, significantly reducing blood flow. PAD predominantly affects the legs, particularly the arteries supplying the thighs and calves. In rare cases, it may involve other arteries, including those in the arms.Etiology of PAD:The principal cause of PAD is atherosclerosis, which results from fatty...