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Effective long range mapping in Schizosaccharomyces pombe with the help of swi5
1Institut für Genetik, Technische Universität Braunschweig, Germany.
Current Genetics
|September 1, 1993
Abstract:
The switching gene swi5 has a function in mating-type switching. In addition, the swi5 mutation causes an increased radiation sensitivity and reduces meiotic recombination about ten-fold. Based on the latter property, an experimental protocol was developed for using swi5 in long-range mapping in S. pombe. It is suitable for a speedy mapping of any new gene which has not yet been cloned. The procedure was used to clarify the map positions of some genes.