[A high affinity CD3 monoclonal antibody HIT3a. I. Production and identification]

A monoclonal antibody (McAb, named HIT3a) against CD3 antigen of human T lymphocytes was produced by the mouse-mouse hybridization technique and identified by immunofluorescent test, immunohistochemistry assay, comparison with standardized McAbs, and some studies of biological properties. HIT3a McAb was found to have high affinity, good stability, complement fixation activity, specific cytotoxicity against T cells, and effective mitogenic activity on normal PBMCs. So it might be a good reagent for clinical and laboratory research.