Related Experiment Video
Updated: Aug 14, 2026

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
Published on: May 7, 2013
[The influence of DPDPE on intracellular free calcium concentration induced by Bt2 cAMP and phorbol ester]
1Institute of Basic Medical Sciences, Beijing.
Abstract:
The effect of DPDPE on intracellular free calcium concentration in neuroblastoma glioma hybrid cells (NG108-15) was studied with fura-2/AM fluorescence. The results were as follows: 1) The membrane permeable cAMP analogue Bt2cAMP, the adenylate cyclase activator forskolin and the protein kinase C activator TPA all induced an increase in intracellular free calcium concentration. 2) Verapamil suppressed the increase of [Ca2+]i induced by Bt2cAMP, forskolin and TPA. 3) DPDPE blocked the increase of [Ca2+]i induced by Bt2cAMP and forskolin, but not that induced by TPA. These results imply that DPDPE suppresses cyclic AMP-dependent protein kinase-induced but not protein kinase C-induced intracellular free calcium.
Related Concept Videos
What are Second Messengers?
Phosphoinositides and PIPs
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Amplifying Signals via Second Messengers
IP3/DAG Signaling Pathway
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

