[The effect of IH764-3 on proliferation and function of fibroblasts]

IH764-3, a potent component isolated from Salviae miltiorrhizae (a component of TML) was used to study the effect on proliferation and functions of cultured fibroblasts. The fibroblast growth curve demonstrated a dose-dependent relationship between growth and IH764-3 concentration. The incorporation of 3H-TdR and 3H-proline into fibroblasts was significantly inhibited by IH764-3. Calmodulin level, fibronectin and thrombospondin contents in the test group were obviously lower than those in the control group. Flow-cytometry showed that in the IH764-3 treated group, the percentage of cells in G0 + G1 phase was higher than that in the control. Electron microscopic observation (TEM and SEM) showed that in the treated group, the secretory function of collagen had decreased. All the results indicated that IH764-3 exerts a direct inhibitory effect on fibroblast proliferation and affects their ability to synthesize and secrete collagenous substances.