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CD21-CD23 ligand pair expression in children with allergic asthma
S Rabatić1, A Gagro, M Medar-Lasić
1Institute of Immunology, Zagreb, Croatia.
Clinical and Experimental Immunology
|November 1, 1993
Summary
Allergic children show increased levels of CD23+ cells and higher expression of CD21 and CD23 receptors on lymphocytes. This suggests potential issues with CD21-CD23 interactions in allergy.
Area of Science:
- Immunology
- Allergy Research
- Cell Biology
Background:
- The CD23 antigen is a low-affinity IgE receptor that interacts with CD21.
- This CD21-CD23 interaction is crucial for T cell-B cell communication and regulating IgE production.
- Dysregulation of these interactions may contribute to allergic conditions.
Purpose of the Study:
- To investigate the expression of CD21 and CD23 on lymphocytes in allergic children compared to non-allergic controls.
- To analyze potential differences in B cell subsets expressing these antigens.
Main Methods:
- Flow cytometry was used to analyze CD21 and CD23 expression on CD4+ and CD20+ lymphocytes.
- Three-color fluorescence analysis was performed to examine B cell subsets.
Main Results:
- Allergic children exhibited significantly higher percentages and absolute numbers of CD23+ cells.
- Increased mean fluorescence intensity of both CD21 and CD23 receptors was observed in allergic children.
- A subset of CD20+ CD21- cells showed elevated CD23 expression in allergic children.
Conclusions:
- The findings indicate altered expression and potential dysregulation of the CD21-CD23 interaction in allergic individuals.
- These molecular changes may play a role in the pathogenesis of allergies.