Conditional mutations in the translational apparatus of Bacillus subtils

Four temperature sensitive mutants of B. subtilis were isolated by localized mutagenesis in the major ribosomal gene cluster, and charcterized genetically and biochemically. Three are mutations which cause temperature sensitivity in the elongation factor Ef-G, and one which has a similar effect on the elongation factor Ef-Tu. They map in a cluster near strA, with the temperature sensitive mutations in Ef-G mapping between the strA gene and the temperature sensitive mutation in Ef-TU.