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Changes in intracellular pH during repeated exercise

T Yoshida1, H Watari

  • 1Exercise Physiology Laboratory, Faculty of Health and Sport Sciences, Osaka University, Japan.

European Journal of Applied Physiology and Occupational Physiology
|January 1, 1993
PubMed
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Repeated exercise causes intracellular pH to become less acidic, with long-distance runners showing less acidity than controls. This was measured using 31 phosphorus-nuclear magnetic resonance spectroscopy (31P-MRS) during femoral flexion exercises.

Area of Science:

  • Exercise Physiology
  • Biophysics
  • Skeletal Muscle Metabolism

Background:

  • Intracellular pH (pHi) changes during exercise are critical for muscle function and fatigue.
  • Understanding pHi dynamics during repeated exercise bouts is essential for optimizing training protocols.

Purpose of the Study:

  • To quantify intracellular pH changes during repeated exercise sessions with intervening rest periods.
  • To compare pHi responses between trained long-distance runners and healthy controls.

Main Methods:

  • Utilized 31 phosphorus-nuclear magnetic resonance spectroscopy (31P-MRS) to measure intracellular pH.
  • Subjects performed repeated 2-minute femoral flexion exercises with 2-minute rest intervals.
  • Analyzed inorganic phosphate (Pi) peak splitting and chemical shifts to determine pH values.

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Main Results:

  • During exercise, inorganic phosphate (Pi) peaks split, indicating distinct high-pH and low-pH compartments.
  • The high-pH Pi area decreased, while the low-pH Pi area increased with repeated exercise bouts.
  • Intracellular pH became less acidic with successive exercise periods, with controls exhibiting greater acidity than runners.

Conclusions:

  • Repeated exercise leads to a less acidic intracellular environment, likely due to metabolic buffering.
  • Long-distance runners demonstrate a more resilient intracellular pH regulation compared to untrained individuals.
  • 31P-MRS is a valuable tool for assessing muscle pH kinetics during dynamic exercise.